Hidden VIM-1 metallo-beta-lactamase phenotypes among Acinetobacter baumannii clinical isolates.

نویسندگان

  • Alexandros Ikonomidis
  • Eleni Ntokou
  • Antonios N Maniatis
  • Athanassios Tsakris
  • Spyros Pournaras
چکیده

A total of 87 Acinetobacter baumannii nonrepetitive consecutive clinical isolates were tested for the presence of metallo-beta-lactamases (MBLs). Results of phenotypic assays (MBL Etest, imipenem/imipenem-EDTA combined-disk test, and imipenem/EDTA double-disk synergy test) were negative in all cases, but molecular testing revealed the presence of two bla(VIM-1)-carrying isolates. One isolate had bla(VIM-1) preceded by a weak P1 promoter, and both had inactivated P2 promoters and reduced bla(VIM-1) expression, partially justifying the results revealing hidden MBL phenotypes.

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Detection of VIM- and IMP-type Metallo-Beta-Lactamase Genes in Acinetobacter baumannii Isolates from Patients in Two Hospitals in Tehran

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Detection of VIM- and IMP-type Metallo-Beta-Lactamase Genes in Acinetobacter baumannii Isolates from Patients in Two Hospitals in Tehran.

BACKGROUND Acinetobacter baumannii, is an opportunistic pathogen and is responsible for numerous nosocomial infections. In recent years, this microorganism has been resistant to a wide range of antibiotics. One of the most important mechanisms of resistance in this microorganism is production of metallo-beta-lactamases (MBLs). OBJECTIVES The aim of this study was to detect VIM- and IMP-type m...

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 46 1  شماره 

صفحات  -

تاریخ انتشار 2008